The dysregulation of lipid metabolism is at the center of several human diseases, including cancer, fatty liver and cardiovascular disease. For this reason we are interested in understanding the molecular mechanism that regulate lipid homeostasis in health and disease, and in recent years have applied genome-wide functional genetic screens to identify novel genes and mechanisms that play a role herein (e.g. Loregger et al. Nat. Comm, 2020).
As part of the European ITN consortia EndoConnect we have a PhD position to further expand the use of whole-genome mammalian haploid- and CRISPR-Cas9-based genetic screens to interrogate lipid metabolism. We especially aim to study the regulation of endogenously tagged (disease-associated) proteins involved in intracellular trafficking of cholesterol within the endo-lysosomal network, and in delivery of lipoprotein-derived cholesterol to the ER/lipid droplets. Identified regulators will be studied using a wide range of modern experimental approaches (including genetic screens, cell-based functional assays, lipidomics, mass spectrometry, imaging, and molecular biology) in advanced cellular and mouse models. The metabolic consequence of hepatic-specific deficiency of identified regulators on lipid and energy metabolism in atherosclerosis and metabolic syndrome will be investigated.
As a PhD student within EndoConnect you will be part of an international team of young researchers. You will have your individual research project at your host organization, and to broaden your PhD training you will undertake internships at complementary EndoConnect partner organizations. In addition, you will follow advanced interdisciplinary courses by leaders in the field of molecular and cell biology, metabolism, physiology and biomedical research.
We are looking for a broadly-trained and highly-motivated PhD candidate with an (almost completed) MSc in Medical Biology, Life Sciences, Biochemistry or a related field. Experience and/or affinity with metabolism, functional genetic screens, next-generation sequencing, or bioinformatics is an advantage. The candidate should have good command of oral and written English. Excellent communication skills and the ability to work within an international research group are essential. The appointment will be for 4 years.
Eligibility criteria: Due to program-specific criteria candidates should:
The Zelcer group is located in the Department of Medical Biochemistry. Information on our research and group can be found on our website: http://www.zelcerlab.eu/.
We offer you ample opportunity for development, deepening and broadening, additional training and a place to grow! Working at Amsterdam UMC means working in an inspiring and professional environment where development is encouraged in every respect.
For an overview of all our other terms of employment, see https://werkenbijamc.nl/arbeidsvoorwaarden/.
Amsterdam UMC has an open culture. Together we continuously build an environment where everyone feels welcome. To achieve that, we strive to provide equal opportunities for everyone. We therefore cordially invite all interested parties to respond to this vacancy.
If you would like to apply directly, please use the ‘apply’ button on this page.
If you would like more information, please feel free to contact Noam Zelcer (Prof. Dr.), via email@example.com.
Applications should include a CV, cover letter, and the contact information of two references.
We look forward to meeting you!
An assessment may be part of the selection procedure.