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Research technician on complex in vitro gut-brain organoid model

Apply before: 16 January 2021
Working time: Day
Working hours: 36 hours per week
Discipline: AMC Medical Research (AMR)

What you are going to do

GUTVIBRATIONS mission is to develop, a next-generation gut-brain axis organ-on-chip for virology. This multi-organ system will provide an animal-free solution for modelling human diseases and pre-clinical drug development.

GUTVIBRATIONS is a multidisciplinary international consortium funded by the European Commission. The consortium consists of 7 esteemed European partners in the fields of academia (Amsterdam UMC, Technical University of Denmark, University of Leuven, KTH Royal Institute of Technology) and industry (UniQure Biopharma BV, BIOLAMINA AB and STEMCELL Technologies UK ltd.). With our team of experts in the fields of organ-on-chip technology, 3D printing, bioengineering, organoid technology, virology, immunology and cell culturing, GUTVIBRATIONS aims to take organ-on-chip technology to the next level in the biomedical industry.

Our consortium is currently looking for 9 postdoctoral researchers, 2 laboratory technicians and 1 PhD student to join our team. Are you looking to be part of an ambitious, driven and passionate team who will lead innovation in the field of organ-on-chip technology for virology? Then check our vacancies and apply! The application deadline for all vacancies is the 15th of January 2021.

GUTVIBRATIONS is divided into Work Packages. The objectives per Work Package are described on the GUTVIBRATIONS website.

Within GUTVIBRATIONS, the Amsterdam UMC is looking for an experienced research technician to work on Work Packages 1,2,3, and 7. The research technician will work together with two postdoctoral researchers on the creation of a gut epithelial-mesenchyme co-culture model. Immune cells will then be added to create a gut-mucosal model. Both models will be used for studying enteric viral infection and antiviral testing. Finally, the gut-mucosal model will be combined with a blood-brain-barrier(BBB)-brain organoid model to create a complex in vitro gut-brain axis organ-on-chip (see www.gutvibrations.org).

The research technician will be supporting the postdoctoral researchers with all the technical aspects of the project.  The most important tasks are the design and fabrication of STACKS plates, supply of STACKS plates to the consortium, supporting the postdoctoral researchers with culture using STACKS, and developing (together with the postdocs) standard operating procedures for cell culture, immunostaining, Biosilk patterning of STACKS plates.

You will be guided during your research project by Dr. Katja Wolthers (Department of Medical Microbiology) and Dr. Dasja Pajkrt (Paediatric Infectious Disease Specialist). In the OrganoVIR Labs under their leadership, pathogenesis of paediatric virus infections in human models is the main topic of research. The individual organoid models that have been established over the past years will lay the foundation for this project

What we expect from you

Amsterdam UMC is looking for a highly driven and experienced research technician who

  • Has a college/HBO degree in Biology and Medical Laboratory Research, possibly supplemented with a Master Degree in lab-on-chip or microfabrication
  • Has experience in embossing, casting, microfabrication of organ-on-chip devices and hydrogel matrixes
  • Has experience with basic cell culture, immunofluorescent staining. Experience with other molecular biology techniques will be advantageous 
  • Is experienced working independently, but is also highly capable (and likes) to work in a team
  • Enjoys collaborating and contributing ideas within the research group
  • Can adapt quickly to new situations and is able to adapt to changes regarding project tasks
  • Is highly proficient in the English language
  • Has excellent communication skills
  • Experience with virology is desirable but not necessary

Where you are going to work

Amsterdam University Medical Centers, location AMC, houses the university hospital and the medical faculty of the University of Amsterdam, as well as several research institutes. Infectious Diseases represents one of the major research topics at the AMC, integrated in a multidisciplinary fashion at the Amsterdam Immunity and Infection Institute (AIII).

Research at the Department of Medical Microbiology broadly covers bacteriology, parasitology, and virology. Laboratories at the department have biosafety facilities up to level 3, state-of-the-art molecular biology and culture facilities and access to high-throughput sequencing. The AMC is currently the coordinator for a Health Holland-PPP Allowance project: Focus-on-Virus and for OrganoVIR, a Marie Sklodowska-Curie Actions Innovative Training Network (ITN) dedicated to the use of organoids for virus research (www.organovir.com).

What we offer you

We offer you ample opportunity for development, deepening and broadening, additional training and a place to grow! Working at AMR means working in an inspiring and professional environment where development is encouraged in every respect.

• The maximum gross monthly salary based on a 36-hour work week is between € 2.769,- and € 3.712,- (scale 8 CAO UMC)
• The base salary does not include holiday pay (8%) and a year-end bonus (8.3%).
• We offer a contract for 48 months.
• In addition to excellent accessibility by public transport, AMC also has a sufficient number of parking spaces for employees.
• Pension is accrued at Be Frank.

For an overview of all our other terms of employment, see https://werkenbijamc.nl/arbeidsvoorwaarden-amr/

Let’s meet

If you would like to apply directly, please apply via www.gutvibrations.org/recruitment/.
If you would like more information, please feel free to contact Dasja Pajkrt (Paediatric Infectious Disease Specialist), via gutvibrations@amsterdamumc.nl

NOTE: All applications must be submitted via the application form on the GUTVIBRATIONS website. We do not consider applications that have not been submitted via the GUTVIBRATIONS website.

We look forward to meeting you!